Generator

Part:BBa_K1179021:Design

Designed by: Lauren St. Hilaire   Group: iGEM13_MIT   (2013-09-15)


CAG_Acyl-TyA-mKate-6xHis fusion protein


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 1730
    Illegal XbaI site found at 1634
    Illegal PstI site found at 2359
    Illegal PstI site found at 2608
    Illegal PstI site found at 2630
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 1730
    Illegal PstI site found at 2359
    Illegal PstI site found at 2608
    Illegal PstI site found at 2630
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 1730
    Illegal BglII site found at 2459
    Illegal XhoI site found at 12
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 1730
    Illegal XbaI site found at 1634
    Illegal PstI site found at 2359
    Illegal PstI site found at 2608
    Illegal PstI site found at 2630
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 1730
    Illegal XbaI site found at 1634
    Illegal PstI site found at 2359
    Illegal PstI site found at 2608
    Illegal PstI site found at 2630
    Illegal NgoMIV site found at 1322
    Illegal NgoMIV site found at 1479
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 3115


Design Notes

Not applicable

Source

Constructed using Gateway reactions with entry vectors CAG and Acyl-TyA-mKate-His. The TyA protein is a yeast viral protein and when added with the Acylation tag it acts as an exosome export protein, allowing the eGFP to be exported when fused to the TyA.

References